Dengue virus infection is prevalent in tropical and sub-tropical regions of the world, which is fatal if untreated symptomatically. Emergence of new genotype within serotypes led to enhanced severity. The objective of the study is to identify the molecular characteristics of the DENV circulated during 2017 outbreak in Tamil Nadu, India and, to investigate the role of inflammatory cytokines in different ‘serotypes’ and in ‘dengue severity’. A total of 135 suspected samples were tested for DENV infection using IgM, IgG and qPCR assay; where 76 samples were positive for DENV and analysed for twelve inflammatory cytokines using ELISA. Serotyping shows 14 DENV-1, 22 DENV-2, 7 DENV-3 and 33 DENV-4, where DENV-4 was predominant. Among seventy-six, forty-two isolates were successfully sequenced for C-prM region and grouped. A lineage shift was observed in DENV-4 genotype. Irrespective of serotypes, IFNγ was significantly elevated than control and in primary infection indicating its ro
National Pork Board
Julian Montoya Lopez, Juan Sanhueza, Carles Vilalta, Cesar Corzo, University of Minnesota | May 11, 2021
Porcine coronaviruses have been present in the U.S. swine industry for several years (Stevenson et al., 2013). Transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV) and porcine Deltacoronavirus (PDCoV) continue to be part of the list of differential diagnosis when facing a clinical case of gastrointestinal disease (Marthaler et al., 2014; Turlewicz-Podbielska & Pomorska-Mól, 2021; Xia et al., 2018; Zimmerman., 2019). Unfortunately, both PEDV and PDCoV are responsible for breaks in breeding herds indicating that the virus continues to transmit within the pig population.
Even though the incidence of both viruses in the breeding herd population remains below 10% (MSHMP, 2021), it is not well understood what the situation is in the growing pig herd. Pig production companies continue to invest resources in order t