Tissue engineered constructs can serve as in vitro models for research and replacement of diseased or damaged tissue. As an emerging technology, 3D bioprinting enables tissue engineering through the ability to arrange biomaterials and cells in pre-ordered structures. Hydrogels, such as alginate (Alg), can be formulated as inks for 3D bioprinting. However, Alg has limited cell affinity and lacks the functional groups needed to promote cell growth. In contrast, graphene oxide (GO) can support numerous cell types and has been purported for use in regeneration of bone, neural and cardiac tissues. Here, GO was incorporated with 2% (w/w) Alg and 3% (w/w) gelatin (Gel) to improve 3D printability for extrusion-based 3D bioprinting at room temperature (RT; 25°C) and provide a 3D cellular support platform. GO was more uniformly distributed in the ink with our developed method over a wide concentration range (0.05%–0.5%, w/w) compared to previously reported GO containing bioink. Cell support w
Collagens from a wide array of animals have been explored for use in tissue engineering in an effort to replicate the native extracellular environment of the body. Marine-derived biomaterials offer promise over their conventional mammalian counterparts due to lower risk of disease transfer as well as being compatible with more religious and ethical groups within society. Here, collagen type I derived from a marine source (Macruronus novaezelandiae, Blue Grenadier) is compared with the more established porcine collagen type I and its potential in tissue engineering examined. Both collagens were methacrylated, to allow for UV crosslinking during extrusion 3D printing. The materials were shown to be highly cytocompatible with L929 fibroblasts. The mechanical properties of the marine-derived collagen were generally lower than those of the porcine-derived collagen; however, the Young’s modulus for both collagens was shown to be tunable over a wide range. The marine-derived collagen was se
Skin provides the protective surface for animals and humans and is therefore prone to physical, chemical, and biological injuries. In all but superficial wounds, the capacity to repair by regeneration is lost and the mechanisms involved in wound closure are unable to restore the skin’s original functions. In this context, skin repair is achieved using surgical techniques including skin grafts, and a range of synthetic or biological scaffolds. Wounds impact millions of patients every year and represent a serious cause of morbidity and mortality worldwide. The increase in need for better skin repair, in part due to issues such as the aging population coupled with chronic conditions has driven the development of products to enhance therapeutic outcomes, yet current treatment outcomes are far from ideal and complete replication of the cellular structure and tissue functional requirements of skin remains a challenge. General aims: Address the major drawbacks of available skin substitutes