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Calibrated adaptive optics improves nanoscale imaging

Calibrated adaptive optics improves nanoscale imaging
optics.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from optics.org Daily Mail and Mail on Sunday newspapers.

Bordeaux Valentin , Stefan Hell , University Of Bordeaux , University Of Bordeaux Interdisciplinary Institute For Neuroscience , Bordeaux Interdisciplinary Institute , ஸ்டீபன் நரகம் , பல்கலைக்கழகம் ஆஃப் போர்டியாக்ஸ் ,

STED microscopy reveals sub-cellular details in living mice


STED microscopy reveals sub-cellular details in living mice
06 Apr 2021
Yale project achieves super-resolution 3D imaging in native tissue environment.
Spot the diference: 3D STED
A group at Yale University has successfully captured 3D super-resolution images from the brains of living mice.
As published in Optica, the breakthrough was based on the use of stimulated emission depletion (STED) microscopy, one of the family of techniques developed to allow imaging beyond the normal diffraction limit of optical microscopy.
The principles behind STED, for which Stefan Hell shared the 2014 Nobel prize for chemistry, involve the addition of a quenching laser pulse to conventional fluorescence excitation, one delivered in a ring-shaped profile. Resolution at the central hole of this pulse can be driven down below the diffraction limit under certain conditions. ....

Stefan Hell , Yale Mary Velasco , Joerg Bewersdorf , Yale University , Yale School Of Medicine , Yale School , ஸ்டீபன் நரகம் , யேல் மேரி வெலாஸ்கோ , யேல் பல்கலைக்கழகம் , யேல் பள்ளி ஆஃப் மருந்து , யேல் பள்ளி ,