Key applications of fluorescence quantification news-medical.net - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from news-medical.net Daily Mail and Mail on Sunday newspapers.
Single-cell multi-omics analysis takes multiple measures from the same individual cell, which provides a more comprehensive picture of cellular diversity and heterogeneity than traditional methods. This approach can also be applied to uncover the molecular mechanisms underlying disease-related processes and reveal new diagnostic markers and therapeutic targets.
Most loved by scientists: 7 top-rated lab products awarded new Seals of Quality at Cancer & Immunology Research Summit 2021
Editorial Article: Most loved by scientists: 7 top-rated lab products awarded new Seals of Quality at Cancer & Immunology Research Summit 2021
From DNA extraction kits to pipettes and imaging systems, find out the latest products to join the exclusive ranks of lab equipment most loved by scientists across the world
14 May 2021
There are four levels of Seal of Quality Platinum, Gold, Silver, Bronze which are allocated to products based on the number of reviews they receive from scientists and the average rating of those reviews
Most loved by scientists: 7 top-rated lab products awarded new Seals of Quality at Cancer & Immunology Research Summit 2021
Editorial Article: Most loved by scientists: 7 top-rated lab products awarded new Seals of Quality at Cancer & Immunology Research Summit 2021
From DNA extraction kits to pipettes and imaging systems, find out the latest products to join the exclusive ranks of lab equipment most loved by scientists across the world
11 May 2021
There are four levels of Seal of Quality Platinum, Gold, Silver, Bronze which are allocated to products based on the number of reviews they receive from scientists and the average rating of those reviews
Using CellDrop to Count Isolated Nuclei
Sponsored Content by DeNovix Inc.Mar 5 2021
The successful isolation of nuclei is an essential step in both ATAC-sequencing workflows and single cell RNA-sequencing. Successful library preparation requires users to ensure that all sample input is non-clustered and debris-free.
Dual fluorescence counting using the CellDrop™ FL Automated Cell Counter is a useful tool, able to distinguish unlysed intact cells from isolated nuclei.
Cluster analysis software provides an aggregation estimate to the user, with the resulting images allowing the user to verify that any debris has been effectively removed.
Counting nuclei with AO/PI stain
Acridine orange and propidium iodide (AO/PI) are utilized to confirm successful nuclei isolation. In conventional cell viability testing, the AO/PI dye combination will stain live cells so that these fluoresce green while staining dead cells so that these fluoresce red.