Quantifying Isolated Nuclei from Mouse Brain Using the CellDrop™ FL Automated Cell Counter
Isolating nuclei is an essential step in both single nuclear RNA-sequencing and ATAC-sequencing workflows.
It is important to ensure that samples obtained during library preparation are non-clustered and debris-free, but nuclei isolated from tissue samples in areas like the brain are prone to high debris in comparison with samples obtained from cultured cells.
It is also important that nuclei are accurately counted for single cell analysis, even in instances where debris does not impact upon library preparation.
The CellDrop™ FL Automated Cell Counter’s dual fluorescence counting capabilities can clearly distinguish debris, nuclei and unlysed intact cells via acridine orange/propidium iodide (AO/PI) counting.