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Pinpointing how cancer cells turn aggressive


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IMAGE: Researchers from the University of Pennsylvania used a novel technique based on the CRIPSR-Cas9 system to precisely track the lineage of cancer cells. They found that that one clone (represented.
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Credit: University of Pennsylvania
It s often cancer s spread, not the original tumor, that poses the disease s most deadly risk.
And yet metastasis is one of the most poorly understood aspects of cancer biology, says Kamen Simeonov, an M.D.-Ph.D. student at the University of Pennsylvania Perelman School of Medicine.
In a new study, a team led by Simeonov and School of Veterinary Medicine professor Christopher Lengner has made strides toward deepening that understanding by tracking the development of metastatic cells. Their work used a mouse model of pancreatic cancer and cutting-edge techniques to trace the lineage and gene expression patterns of individual cancer cells. They found a spectrum of aggression in the cells that a ....

Beth Martin , Jay Shendure , Kamen Simeonov , Meganl Clark , Christopher Lengner , Robertj Nogard , Aaron Mckenna , Benz Stanger , Shipley Foundation Program For Innovation , University Of Pennsylvania Perelman School Medicine , University Of Pennsylvania School Veterinary Medicine , National Institutes Of Health , School Of Veterinary Medicine , Perelman School Of Medicine , University Of Washington , School Of Arts Sciences , Dartmouth Geisel School Of Medicine , Blavatnik Family Fellowship In Biomedical Research , Allen Discovery Center , Penn Medical Science Training Program , Howard Hughes Medical Institute , Department Of Biomedical Sciences , Pennsylvania Perelman School , Veterinary Medicine , Cancer Cell , Biomedical Sciences ,

Novel integrative genetic screening platform offers insights into role of DNA accessibility in cancer


Novel integrative genetic screening platform offers insights into role of DNA accessibility in cancer
In a new resource for the scientific community, published today in
Nature Biotechnology, researchers in the lab of Neville Sanjana, PhD, at the New York Genome Center (NYGC) and New York University (NYU) developed CRISPR-sciATAC, a novel integrative genetic screening platform that jointly captures CRISPR gene perturbations and single-cell chromatin accessibility genome-wide.
With this technology, they profile changes in genome organization and create a large-scale atlas of how loss of individual chromatin-altering enzymes impacts the human genome. The new method harnesses the programmability of the gene editing system CRISPR to knock-out nearly all chromatin-related genes in parallel, offering researchers deeper insights into the role of DNA accessibility in cancer and in rare diseases involving chromatin. ....

New York , United States , New York University , Neville Sanjana , Noa Liscovitch Brauer , Jay Shendure , Emily Henderson , Sanjana Lab , Innovation Technology Lab , York Genome Center , University Of Washington , Grossman School Of Medicine , Study Co , York University , Nature Biotechnology , New York Genome Center , Study Co Author , Postdoctoral Fellow , Antonino Montalbano , Core Faculty Member , Assistant Professor , புதியது யார்க் , ஒன்றுபட்டது மாநிலங்களில் , புதியது யார்க் பல்கலைக்கழகம் , நெவில் சஞ்சனா , ஜெய் ஷெண்டுரே ,

Single-cell CRISPR technology deciphers role of chromatin accessibility in cancer


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IMAGE: CRISPR-sciATAC is a novel integrative genetic screening platform that jointly captures CRISPR gene perturbations and single-cell chromatin accessibility genome-wide. The new method harnesses the programmability of the gene editing system.
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Credit: Sanjana Lab of New York Genome Center and NYU
NEW YORK, NY (April 29, 2021) - In a new resource for the scientific community, published today in
Nature Biotechnology, researchers in the lab of Neville Sanjana, PhD, at the New York Genome Center (NYGC) and New York University (NYU) developed CRISPR-sciATAC, a novel integrative genetic screening platform that jointly captures CRISPR gene perturbations and single-cell chromatin accessibility genome-wide. With this technology, they profile changes in genome organization and create a large-scale atlas of how loss of individual chromatin-altering enzymes impacts the human genome. The new method harnesses the programmability of the gene ed ....

New York , United States , Memorial Sloan Kettering Cancer Center , Rockefeller University , New York University , Cold Spring Harbor Laboratory , Hospital For Special Surgery , American Museum Of Natural History , Columbia University , Neville Sanjana , Noa Liscovitch Brauer , Karen Zipern , Jay Shendure , Sanjana Lab , York Genome Center , Rutgers Cancer Institute Of New Jersey , University Of Washington , Grossman School Of Medicine , Roswell Park Cancer Institute , York University , Hackensack Meridian Health , York Stem Cell Foundation , Northwell Health , Albert Einstein College Of Medicine , Princeton University , Stony Brook University ,