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New Nanopore Technique Captures Cell-Free DNA from Sample Surfaces

New Nanopore Technique Captures Cell-Free DNA from Sample Surfaces
Source: seksan Mongkhonkhamsao/Getty Images
April 7, 2021
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Scientists from the University of California-Riverside say they have found a way to better detect and capture DNA from fluid samples such as blood using a tiny glass tube and electric current. The technique (“Measuring trapped DNA at the liquid-air interface for enhanced single molecule sensing”), described in
Nanoscale, can also improve cancer diagnosis in the future, according to the team.
“Nanopore sensing is a promising tool with widespread application in single-molecule detection. Borosilicate glass nanopores are a viable alternative to other solid-state nanopores due to low noise and cost-efficient fabrication. For dielectric materials, including borosilicate glass, the capacitive noise is one of the major contributors to noise, which depends on the wall thickness and the surface area submerged in an ionic solution ....

United States , Kevin Freedman , University Of California , Rosemary Bourns College Of Engineering , Rosemary Bourns College , ஒன்றுபட்டது மாநிலங்களில் , கெவின் விடுவிக்கப்பட்டவர் , பல்கலைக்கழகம் ஆஃப் கலிஃபோர்னியா , ரோஸ்மேரி பெர்ன்ஸ் கல்லூரி ஆஃப் பொறியியல் , ரோஸ்மேரி பெர்ன்ஸ் கல்லூரி ,

New Nanopore Technique Captures Cell-Free DNA from Biological Samples

New Nanopore Technique Captures Cell-Free DNA from Biological Samples
Written by AZoNanoApr 7 2021
Although DNA sequencing has now turned more common, very few understand how difficult it is to extract even a single molecule of DNA from a biological sample.
An illustration showing (a) how a glass tube with a tiny opening and a positive electrode, when inserted into a liquid sample and stimulated with electricity, collects cell-free DNA floating in the sample; (b) a photograph of the borosilicate glass nanopore. Image Credit: Freedman et al., 2021.
Led by UC Riverside, a new study is enabling easy detection and capture of DNA from fluid samples like blood with a tiny glass tube and electric current. The technique has been explained in the ....

Kevin Freedman , Laurens Lastra , Vinay Sharma , Nasim Farajpour , Rosemary Bourns College Of Engineering , Rosemary Bourns College , Assistant Professor , கெவின் விடுவிக்கப்பட்டவர் , லாரன்ஸ் லஸ்ற்ர , வினய் ஷர்மா , ரோஸ்மேரி பெர்ன்ஸ் கல்லூரி ஆஃப் பொறியியல் , ரோஸ்மேரி பெர்ன்ஸ் கல்லூரி , உதவியாளர் ப்ரொஃபெஸர் ,

Glass nanopore pulls DNA like spaghetti through a needle

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DNA sequencing has become so common, few realize how hard it is to even extract a single molecule of DNA from a biological sample.
Research led by UC Riverside is making it easier to detect and capture DNA from fluid samples such as blood using a tiny glass tube and electric current. The technique, described in the journal,
Nanoscale, can also improve cancer diagnosis in the future.
DNA, a double-stranded, electrically charged molecule that contains all the information an organism needs to create and organize the building blocks of life, is tightly folded within the cell nucleus. Extracting the DNA from a single cell is time consuming and impractical for many medical and scientific purposes. Fortunately, as cells die naturally, their membranes burst, releasing the contents, including DNA. This means that a blood sample, for example, contains many strands of free-floating DNA that should, in theory, be easier to identify and extract in quantity. ....

Kevin Freedman , Laurens Lastra , Nasim Farajpour , Vinay Sharma , Rosemary Bourns College Of Engineering , Rosemary Bourns College , கெவின் விடுவிக்கப்பட்டவர் , லாரன்ஸ் லஸ்ற்ர , வினய் ஷர்மா , ரோஸ்மேரி பெர்ன்ஸ் கல்லூரி ஆஃப் பொறியியல் , ரோஸ்மேரி பெர்ன்ஸ் கல்லூரி ,