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Discovering DNA methylation from bacteria and microbiome using nanopore technology

Discovering DNA methylation from bacteria and microbiome using nanopore technology
Journal name:
Nature Methods
Title of the article: Discovering multiple types of DNA methylation from individual bacteria and microbiome using nanopore sequencing
Corresponding author: Gang Fang, PhD
Bottom line:
Bacterial DNA methylation occurs at diverse sequence contexts and plays important functional roles in cellular defense and gene regulation. An increasing number of studies have reported that bacterial DNA methylation has important roles affecting clinically relevant phenotypes such as virulence, host colonization, sporulation, biofilm formation, among others.
Bacterial methylomes contain three primary forms of DNA methylation: N6-methyladenine (6 mA), N4-methylcytosine (4mC) and 5-methylcytosine (5mC). The widely used bisulfite sequencing for DNA methylation mapping in mammalian genomes are not effective at resolving bacterial methylomes. Single molecule real-tim ....

Emily Henderson , Gang Fang , Mount Sinai , Dna Methylation , எமிலி ஹென்டர்சன் , கும்பல் ஃப்யாஂக் , ஏற்ற சினை ,

DNA methylation from bacteria & mircobiome using nanopore technology discovered

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IMAGE: Gang Fang, PhD, Associate Professor, Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai
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Credit: Mount Sinai Health System
Journal Name:
Nature Methods
Title of the Article: Discovering multiple types of DNA methylation from individual bacteria and microbiome using nanopore sequencing
Corresponding Author: Gang Fang, PhD
Bottom Line:
Bacterial DNA methylation occurs at diverse sequence contexts and plays important functional roles in cellular defense and gene regulation. An increasing number of studies have reported that bacterial DNA methylation has important roles affecting clinically relevant phenotypes such as virulence, host colonization, sporulation, biofilm formation, among others. ....

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China Marks the Wuhan Lockdown Anniversary Amid Spiraling COVID-19 Cases and With Risky Holiday Travel Looming

China Marks the Wuhan Lockdown Anniversary Amid Spiraling COVID-19 Cases and With Risky Holiday Travel Looming
Time
1/22/2021
Charlie Campbell / Changsha and Wuhan
© HECTOR RETAMAL/AFP via Getty Images
A woman walks along a street near a market in Wuhan, China s central Hubei province on January 19, 2021.
When authorities came to Dr. Liu Chun’s hospital in the central Chinese city of Changsha with a request for 130 volunteers, it took just two hours for all slots to be filled. As a respiratory doctor specializing in ICU patients, Liu felt it was her duty to join the group of medical workers summoned 340 kilometers north, to Wuhan, where rumors of a mysterious pneumonia-like illness had been circulating for weeks. At first, Liu, 48, wasn’t terribly worried. Her husband and 12-year-old daughter were supportive; she didn’t bother telling her elderly parents of her plans. ....

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