Identification PH Animal Model
In order to study the pathogenesis of PH, we constructed the PH animal model. After establishing a PH model, we measured the RV/(LV + S), mPAP, WT, and WA (n=6). Compared to the control group, these indexes started to increase 2 weeks after establishing the model and reached their maximum at 4 weeks. These findings indicated the successful establishment of the PH model (Figure 1A). We examined the expression of PTBP1 in pulmonary arterial tissue of rats with PH by Western blot and immunofluorescence assays. The results showed that the expression of PTBP1 was significantly increased 3 weeks after hypoxia (Figure 1B). Then, we evaluated the expression of PTBP1 of PASMCs using Western blot and immunofluorescence. The results indicated that hypoxia significantly promoted the expression PTBP1 after 24h (Figure 1C and D).