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Antibacterial effects of Disulfiram in Helicobacter pylori


Confirmation of Gene Mutation
For all 12
H. pylori strains used, the sequence of 16SrDNA, a ribosomal gene involved in protein production, was analyzed, and the presence or absence of gene mutation was examined. Table 3 shows the number of base substitutions and the number of deletions/insertions in the 16SrDNA sequence. Of these, susceptible strain 3 (S3), amoxicillin-resistant strain 1 (AC1), amoxicillin-resistant strain 2 (AC2), clarithromycin-resistant strain 1 (CH1), and metronidazole-resistant strain 2 (MZ2) were resistant to disulfiram in the growth suppression test. Strains resistant to disulfiram tended to have fewer base deletions/insertions.
Table 3 16S rDNA Sequence
Discussion
Eradication therapy using amoxicillin and clarithromycin is performed for ....

Eteläuomen Läi , Okayama University Ethics Committee Number , Okayama University Hospital , Santa Cruz Biotechnology Inc , Hokkaido System Science Co Ltd , Okayama University Ethics Committee , Growth Inhibition Test , Santa Cruz Biotechnology , Western Blotting Substrate , Thermo Fisher Scientific , Amersham Imager , Nuclei Lysis Solution , Chain Reaction Detection , Kara Ex Taq , Applied Biosystems , Pathogenic Protein Expression Level , ஒக்யமா பல்கலைக்கழகம் மருத்துவமனை , சாந்தா குரூஸ் உயிரி தொழில்நுட்பவியல் இன்க் , சாந்தா குரூஸ் உயிரி தொழில்நுட்பவியல் , தெர்மோ மீனவர் அறிவியல் , அமர்ஷாம் இமேஜர் , பயன்படுத்தப்பட்டது உயிர் அமைப்புகள் ,