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FSTL3 is a prognostic biomarker in gastric cancer


DSCAM-AS1 and miR-122-5p.
Although there has been a small amount of research on FSTL3, the exact mechanism by which
FSTL3 functions as an oncogene remains unclear to date. Therefore, in the present study, we aimed to explore the molecular mechanism of
FSTL3 involvement in gastric carcinogenesis and development, and to evaluate its value as a prognostic biomarker and potential therapeutic target for GC.
Materials and Methods
Cell Culture
AGS (moderately differentiated GC cells), HGC-27 (undifferentiated GC cells), GES-1 (healthy gastric epithelial cells), and THP-1 cells (human monocytic cells) were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China). MKN-74 and MKN-45 cells (well-differentiated and poorly differentiated GC cells, respectively) were purchased from the Japanese Collection of Research Bioresources Cell Bank. GC cells were cultured in RPMI-1640 medium (Gibco, USA, Lot: 8121248) supplemented with 10% fetal bovine ....

United States , United Kingdom , American Joint Committee On Cancer , Graphpad Software Inc , Antibody Sampler Kit Cell Signaling Technology , Aaf Association , Chinese Academy Of Sciences Shanghai , Japanese Collection Of Research Bioresources Cell Bank , Genechem Corporation Shanghai , Colorful Gene Biological Technology , National Comprehensive Cancer Network , Chinese Academy , Japanese Collection , Research Bioresources Cell , Vector Construction , Genechem Corporation , Beyotime Biotechnology , Sampler Kits , Sampler Kit , Cell Signaling Technology , Zhongshan Golden Bridge Biotechnology , Linked Immunosorbent Assay , Jiangsu Provincial Hospital , Traditional Chinese , American Joint Committee , Jiangsu Province Hospital ,