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30 values of each chemotherapeutic agent were applied in the further drug–drug interaction study (cisplatin: 10 μM for HeLa, 12 μM for SiHa; paclitaxel: 0.01 μM for HeLa, 0.006 μM for SiHa; 5-fluorouracil: 0.5 μM for HeLa, 50 μM for SiHa; doxorubicin: 0.075 μM for HeLa, 0.1 μM for SiHa). Figure 1 The effects of quercetin and cisplatin on the viability of cervical cancer cells. ( A and B) HeLa and SiHa were exposed to quercetin with various concentrations (0, 10, 20, 50, 100, 150 and 200 μM) for 24 h and 48 h, respectively; ( C and D) HeLa and SiHa were exposed to cisplatin with various concentrations (0, 10, 20, 50, 100 μM) for 24 h and 48 h, respectively; ( E) HeLa was exposed to combination of various concentrations of quercetin (0, 7.5 and 15 μM) and 10 μM cisplatin for 24h; (

United statesBio tekCell signaling technologyNational institutes of healthBeckman coulterShanghai cell biology medical research instituteChinese academy of sciencesChinese academySubstrate kitZhongshan goldenbridgeCell countingMicroplate readerBiotek instrumentsPhosphate buffered salineBicinchoninic acidProtein assay