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UMass Medical School receives $2.8 million grant for advanced cryo-EM microscope

UMass Medical School receives $2.8 million grant for advanced cryo-EM microscope
umassmed.edu - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from umassmed.edu Daily Mail and Mail on Sunday newspapers.

United States , Andrei Korostelev , Celia Schiffer , Kenneth Turner , Rogerj Davis , Chen Xu , H Arthur Smith , Gladys Smith Martin , Cancer Research , Massachusetts Life Sciences Center , Umass Medical School , Research Infrastructure , Howard Hughes Medical Institute , Institute For Drug Resistance , Medical School , Thermo Fisher , Smith Chair , Gladys Smith Martin Chair , Drug Resistance , Howard Hughes Medical , Titan Krios , Thermo Fisher Scientific Glacios , Novel Therapeutics Delivery , Amed News , ஒன்றுபட்டது மாநிலங்களில் , செலியா ஸ்கிஃபர் ,

Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity

SARS-CoV-2 variants with multiple spike mutations enable increased transmission and antibody resistance. Here, we combine cryo-EM, binding and computational analyses to study variant spikes, including one that was involved in transmission between minks and humans, and others that originated and spread in human populations. All variants showed increased ACE2 receptor binding and increased propensity for RBD up states. While adaptation to mink resulted in spike destabilization, the B.1.1.7 (UK) spike balanced stabilizing and destabilizing mutations. A local destabilizing effect of the RBD E484K mutation was implicated in resistance of the B.1.1.28/P.1 (Brazil) and B.1.351 (South Africa) variants to neutralizing antibodies. Our studies revealed allosteric effects of mutations and mechanistic differences that drive either inter-species transmission or escape from antibody neutralization. ....

New York , United States , United Kingdom , South Africa , Leica Microsystems , Joshua Mendez , Led Eng , Mahira Aragon , Eugene Chua , Rr Core Team , Duke Research Computing , National Institute Of Allergy , Duke Regional Biocontainment Laboratory , Electron Microscopy Sciences , Simons Foundation , Ge Healthcare , National Center , Defense Advanced Projects Agency , National Institutes Of Health , Sera Care Life Sciences , York Structural Biology Center , Common Fund Transformative High Resolution Cryo , Foundation For Statistical Computing , Simons Electron Microscopy Center , Duke University , C Terminal Twinstrep ,

Structure of the merozoite surface protein 1 from Plasmodium falciparum

Escherichia coli and purified under GMP-compatible conditions by Biomeva GmbH (Heidelberg, Germany) using the
P. falciparum MSP-1D construct described by Kauth
et al. (
28). Briefly, the coding sequence of MSP-1D is largely based on the
P. falciparum strain 3D7, but the N-terminal signal peptide and the C-terminal GPI anchor have been removed. The coding sequence was split into two halves (p83/30 and p38/42); each was expressed individually in
E. coli W3110-Z1 and recovered as inclusion bodies. The recombinant MSP-1D was reconstituted as a heterodimer (hdMSP-1) in a process called “pulse renaturation” (
39). The protein was purified using ion exchange and hydroxyapatite chromatography followed by dialysis into phosphate-buffered saline (PBS) and lyophilization. MSP-1 produced in this way was previously shown to assemble in the same manner as the native MSP-1 complex ( ....

Biomeva Gmbh Heidelberg , Max Planck Institute Of Biophysics , Ge Healthcare , Biomeva Gmbh , Amicon Ultra , Tecnai Spirit , Fischione Instruments , Titan Krios , Thermo Fisher Scientific , Max Planck Institute , கே சுகாதாரம் , தெர்மோ மீனவர் அறிவியல் ,

Ship & Bunker Begins Publication of LNG Bunker Prices in Rotterdam

Ship & Bunker Begins Publication of LNG Bunker Prices in Rotterdam
by Ship & Bunker News Team
Thursday May 13, 2021
Leading marine fuels publication
Rotterdam effective immediately.
Titan LNG.
The prices include delivery and are based on an underlying front month contract.
As well as being expressed in terms of a quantity of LNG, like the LNG bunker price indications Ship & Bunker already publishes for
Vancouver, the Rotterdam LNG bunker price indications are also expressed in energy equivalent terms for fuel oil bunkers and MGO.
LNG-380e is the price for an amount of LNG that delivers the energy equivalent of one metric tonne of ....

British Columbia , Martyn Lasek , Bunker Begins Publication , Image Credit , Flip Dankelman , Risk Manager , Managing Director , Titan Krios , Green Zeebrugge , Alternative Marine Power , Uture Fuels All , Nc Hydrogen , Lng Bunkers , Riginals Ampb Content , Op Stories Use This To Put In The Box On Homapge , Unker Fuel , Unker Prices , Unker Indications , Arine Fuel , Unker News , Arine News , Hipping News , பிரிட்டிஷ் கொலம்பியா , மார்டின் ளாசேக் , படம் கடன் , ஆபத்து மேலாளர் ,

The neutralizing antibody, LY-CoV555, protects against SARS-CoV-2 infection in nonhuman primates

Among the most promising therapeutic options for individuals with coronavirus disease 2019 (COVID-19) are monoclonal antibodies (mAbs). In this study, Jones et al . identified, characterized, and tested one such mAb, LY-CoV555, in vitro and in vivo. They found that LY-CoV555 bound to the severe acute respiratory distress syndrome coronavirus-2 (SARS-CoV-2) spike protein and prevented its interaction with angiotensin-converting enzyme 2. Prophylactic treatment with LY-CoV555 protected the upper and lower respiratory tracts of nonhuman primates from becoming infected with SARS-CoV-2. Together, these data support the clinical use of LY-CoV555 for treating patients with COVID-19.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) poses a public health threat for which preventive and therapeutic agents are urgently needed. Neutralizing antibodies are a key class of therapeutics that may bridge widespread vaccination campaigns and offer a treatment solution in populations le ....

South Africa , United States , United Kingdom , K Huntington Eli Lilly , Gatan Solarus , Isoplates Perkinelmer , Eli Lilly , Oxford Cryosystems , Carterra Epitope , Us Department Of Defense , National Institute Of Allergy , Grossman School Of Medicine Startup , Lilly Research Laboratories Collaborative Access Team , Burroughs Wellcome Fund Postdoctoral Enrichment Program Award , Us Department Of Energy , International Patent Application No , Electron Microscopy Sciences , Office Of Science , Committee Of Bioqual Inc , Argonne National Laboratory , Centers For Disease , European Union Horizon , Laboratory Animals , Abcellera Biologics Inc , National Institutes Of Health , P Sipahimalani Abcellera Biologics Inc ,