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Deeper proteome coverage and faster throughput for low input samples on the Thermo Scientific Orbitrap Astral mass spectrometer

In recent years there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts (e.g., single-cells) requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. Traditional proteomics approaches read out the ....

Application Note , Thermo Scientific Orbitrap Astral , Orbitrap Astral , Thermo Fisher Scientific , Thermo Scientific , Application Notes , Scientific Posters , Case Studies , Application Articles , Laboratory Methods , Food And Beverage , Clinical Diagnostics , Life Science , Drug Discovery ,

Automated image analysis reduces user-to-user variability in flow cytometry gating strategies

The need for high-throughput sample acquisition and automation in flow cytometry creates demand for new methods for analytical standardization and for reducing sources of variability. When performed on the Attune CytPix Flow Cytometer, automated image analysis combined with conventional flow cytometry provides a robust quantitative method for standardizing flow cytometry data analysis. In this application note, Thermo Fisher Scientific demonstrates that automated image-assisted analysis enabl ....

Application Note , Attune Cytpix Flow Cytometer , Thermo Fisher Scientific , Application Notes , Scientific Posters , Case Studies , Application Articles , Laboratory Methods , Food And Beverage , Clinical Diagnostics , Life Science , Drug Discovery ,

Utilizing 250 mL sample volumes for EPA method 1664B

EPA Method 1664B was developed for measuring oil and grease in wastewater with Hexane Extractable Material (HEM) concentrations ranging from 5 to 1000 mg/L. However, samples containing high oil and grease content (> 500 mg/L HEM) are challenging to extract and therefore the method allows for a smaller sample to be collected. To run reduced sample volumes, laboratories must be able to demonstrate the ability to meet the method detection limit (MDL) require ....

Application Note , Hexane Extractable Material , Application Notes , Scientific Posters , Case Studies , Application Articles , Laboratory Methods , Food And Beverage , Clinical Diagnostics , Life Science , Drug Discovery ,