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A team of ingenious bioengineers at Arizona State University (ASU) has harnessed the power of childhood nostalgia, unveiling a creative solution to a long-standing challenge in DNA origami research. ....
In this interview, AZoNano speaks with Ebbe Sloth Anderson about a new software-based 'RNA origami' technique that can be used to design RNA molecules that fold into compact and rigid shapes. ....
Biomolecular complexes can form stable assemblies yet can also rapidly exchange their subunits to adapt to environmental changes. Simultaneously allowing for both stability and rapid exchange expands the functional capacity of biomolecular machines and enables continuous function while navigating a complex molecular world. Inspired by biology, we design and synthesize a DNA origami receptor that exploits multivalent interactions to form stable complexes that are also capable of rapid subunit exchange. The system utilizes a mechanism first outlined in the context of the DNA replisome, known as multisite competitive exchange, and achieves a large separation of time scales between spontaneous subunit dissociation, which requires days, and rapid subunit exchange, which occurs in minutes. In addition, we use the DNA origami receptor to demonstrate stable interactions with rapid exchange of both DNA and protein subunits, thus highlighting the applicability of our approach to arbitrary molecu ....