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Epigenetic Enrichment of A549 Lung Cancer Stem Cells


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1Department of Pathology, Science and Education Department of the Fifth People’s Hospital of Qinghai, Xining, Qinhai, 810000, People’s Republic of China;
2Department of Pediatrics, The Fifth Affiliated Hospital of Harbin Medical University, Daqing, Heilongjiang, 163319, People’s Republic of China;
3Department of Nuclear Medicine, The Fifth People’s Hospital of Qinghai, Xining, Qinhai, 810000, People’s Republic of China;
4Department of Physiology, College of Life Science and Biopharmaceutics of Shenyang Pharmaceutical University, Shenyang, Liaoning, 110001, People’s Republic of China;
5Department of Pathology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning, 110001, People’s Republic of China
Correspondence: Mumu Shi
Department of Pathology, Science and Education Department of the Fifth People’s Hospital of Qinghai, Xining, Qinhai, 810000, People’s Republic of China ....

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[Full text] Sinomenine Inhibits the Growth of Ovarian Cancer Cells Through the Sup


CDK1,
BUB1) play important roles in the progression of G2/M phase in the cell cycle.
30,31 Furthermore, real-time PCR analysis was used to verify the results of transcriptome sequencing and the results indicated that the expression trends of the top 18 ranked degree genes were consistent with those obtained by RNA-seq, suggesting that the RNA-seq data reliably reflected the gene expression alterations (Figure 4B). According to the RNA-seq and real-time PCR results, the expressions of cell cycle regulated genes, such as
CDK1,
PLK1, and
BUB1, were significantly decreased after sinomenine treatment. These results were also in accord with the cell phenotype experiments. ....

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