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Metabolic engineering of Thermoanaerobacterium AK17 for increased ethanol production in seaweed hydrolysate | Biotechnology for Biofuels and Bioproducts

Sustainably produced renewable biomass has the potential to replace fossil-based feedstocks, for generation of biobased fuels and chemicals of industrial interest, in biorefineries. In this context, seaweeds contain a large fraction of carbohydrates that are a promising source for enzymatic and/or microbial biorefinery conversions. The thermoanaerobe Thermoanaerobacterium AK17 is a versatile fermentative bacterium producing ethanol, acetate and lactate from various sugars. In this study, strain AK17 was engineered for more efficient production of ethanol by knocking out the lactate and acetate side-product pathways. This was successfully achieved, but the strain reverted to acetate production by recruiting enzymes from the butyrate pathway. Subsequently this pathway was knocked out and the resultant strain AK17 M6 could produce ethanol close to the maximum theoretical yield (90%), leading to a 1.5-fold increase in production compared to the wild-type strain. Strain AK17 was also shown ....

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Biomass Analytics Facility Manager job with Michigan State University - Great Lakes Bioenergy Research Center

Biomass Analytics Facility Manager job with Michigan State University - Great Lakes Bioenergy Research Center
acs.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from acs.org Daily Mail and Mail on Sunday newspapers.

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Improved Carbohydrate Analysis with Pulsed Amperometric Detection (PAD) - - Antec Scientific´s ALEXYS Carbohydrate Analyzer – the workhorse for your laboratory

Improved Carbohydrate Analysis with Pulsed Amperometric Detection (PAD) - - Antec Scientific´s ALEXYS Carbohydrate Analyzer – the workhorse for your laboratory
chemeurope.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from chemeurope.com Daily Mail and Mail on Sunday newspapers.

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