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Validate the accuracy of your liquid handlers

Next Advance overviews the Checkit Go for validating accuracy of liquid handling equipment ....

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Frontiers | Glucocorticoid Receptor-Regulated Enhancers Play a Central Role in the Gene Regulatory Networks Underlying Drug Addiction

Substance abuse and addiction represent a significant public health problem that impacts multiple dimensions of society, including healthcare, the economy, and the workforce. In 2021, over 100,000 drug overdose deaths were reported in the US, with an alarming increase in fatalities related to opioids and psychostimulants. Understanding the fundamental gene regulatory mechanisms underlying addiction and related behaviors could facilitate more effective treatments. To explore how repeated drug exposure alters gene regulatory networks in the brain, we combined capped small (cs)RNA-seq, which accurately captures nascent-like initiating transcripts from total RNA, with Hi-C and single nuclei (sn)ATAC-seq. We profiled initiating transcripts in two addiction-related brain regions, the prefrontal cortex (PFC) and the nucleus accumbens (NAc), from rats that were never exposed to drugs or were subjected to prolonged abstinence after oxycodone or cocaine intravenous self-administration (IVSA). In ....

United States , San Diego , Lisa Maturin , University Of California , National Institutes Of Health , Genomics Center , Conversion Software Illumina , College Of Wisconsin , Diversity Program , Library Preparation , University Of Tennessee Health Science Center , Novogen Inc , Nuclei Library Preparation , Transcribed Regulatory Elements , Supplementary Table , Supplementary Figure , Transcriptional Start Regions , Region Specificity , Transcriptional Start Site Regions , Naive Rats Reveals Activated , Transcriptional Start Sites , Delos Santos , Type Specificity , Associated With Addiction Like , Supplementary Figures , Solberg Woods ,