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Base Editing Delivery Upgrade Using Virus-Like Particles

Scientists at Harvard University have engineered virus-like particles that overcome three bottlenecks to protein delivery that reduce the efficacy of VLP-mediated protein delivery: cargo release, cargo packaging and localization, and VLP component stoichiometry. The study showed the new mode of DNA- and virus-free delivery efficiently transports base editor proteins and guide RNAs to target cells in multiple organs with minimal off-target editing in live newborn and adult mice. ....

United States , Perkin Elmer , David Liu , Jennifer Harbottle , Jyoti Jaiswal , Sekar Kathiresan , Broad Institute , Harvard University , Genetic Medicine Research At Children National , Genetic Medicine Research ,

Gene therapy developed to treat limb-girdle muscular dystrophy

Gene therapy developed to treat limb-girdle muscular dystrophy
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District Of Columbia , United States , Jyoti Jaiswal , Holly Barker , Children National Hospital , National Hospital , Clinical Investigation ,

Gene Therapy for a Rare Muscular Dystrophy Overcomes Earlier Roadblocks

A preclinical study provides proof-of-concept evidence for a new gene therapy approach that offers a potential long-term treatment for limb-girdle muscular dystrophy 2B. Researchers at Children’s National have developed the first non-muscle targeted gene therapy that enhances diseased muscle fiber repair and improves muscle function through a single low dose of an AAV vector that produces human acid sphingomyelinase, released by muscle cells immediately following muscle injury. ....

United States , Sreetama Sen Chandra , Jyoti Jaiswal , Daniel Bittel , Jyotik Jaiswal , Eric Hoffmann , Bradley Williams , Children National , Genetic Medicine Research At Children National , Binghamton University , Children National Hospital , Jain Foundation , George Washington University School Of Medicine , Health Sciences , National Hospital , Clinical Investigation , Genetic Medicine Research , National Health System , George Washington University School , Sen Chandra ,

Managing Calcium Overload Identified as Novel Therapeutic Approach in Treating Injured Muscle Fibers


Managing Calcium Overload Identified as Novel Therapeutic Approach in Treating Injured Muscle Fibers
March 16, 2021
Mutations in a calcium ion channel anoctamin 5 (ANO5), commonly found in muscle cells, cause the debilitating disease called limb girdle muscular dystrophy 2L (LGMD2L). Characterized by progressive adult-onset muscle weakness, LGMD2L patients are often asked to “keep mobile” with no specific guidelines on the type or intensity of activities but weakness in muscles of the calves, thighs, and arms makes it difficult for patients to tolerate physical activity.
“There are no therapies available,” said Jyoti Jaiswal, PhD, principal investigator at Children’s National Hospital’s Center for Genetic Medicine Research. ....

Jyoti Jaiswal , Goutam Chandra , Children National Hospital Center , Genetic Medicine Research , National Hospital , Genetic Medicine , Cell Biology , ஜையோடீ ஜெய்ஸ்வால் , கௌதம் சந்திரா , குழந்தைகள் தேசிய மருத்துவமனை மையம் , ஜெநெடிக் மருந்து ஆராய்ச்சி , தேசிய மருத்துவமனை , ஜெநெடிக் மருந்து , செல் உயிரியல் ,