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Microscope system sharpens scientists' view of neural circuit connections

A newly described technology improves the clarity and speed of using two-photon microscopy to image synapses in the live brain. ....

Josiah Boivin , Yi Xue , Elly Nedivi , Lindar Young , Cognitive Sciences , Picower Institute For , Picower Institute ,

Microscopy technique makes finer images of deeper tissue, more quickly


Microscopy technique makes finer images of deeper tissue, more quickly
July 7, 2021MIT
To create high-resolution, 3D images of tissues such as the brain, researchers often use two-photon microscopy, which involves aiming a high-intensity laser at the specimen to induce fluorescence excitation. However, scanning deep within the brain can be difficult because light scatters off of tissues as it goes deeper, making images blurry.
Two-photon imaging is also time-consuming, as it usually requires scanning individual pixels one at a time. A team of MIT and Harvard University researchers has now developed a modified version of two-photon imaging that can image deeper within tissue and perform the imaging much more quickly than what was previously possible. ....

Murat Yildirim , Cheng Zheng , Josiah Boivin , Dushann Wadduwage , Mriganka Sur , Yi Xue , National Institutes Of Health , Imaging At Harvard University , National Institute Of Biomedical Imaging , Hamamatsu Corporation , Harvard University , Samsung Advanced Institute Of Technology , John Harvard Distinguished Science Fellowship Program , Alliance For Research , Jong Kang Park , John Harvard Distinguished Science Fellow , Advanced Imaging , Newton Professor , National Institutes , National Institute , Biomedical Imaging , Independence Award , Samsung Advanced Institute , John Harvard Distinguished Science Fellowship , செங் ஜெங் , மிரகங்க சூர் ,