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Modification of the Folmer primers for the cytochrome c oxidase gene facilitates identification of mosquitoes | Parasites & Vectors

Accurate identification of mosquito species is essential for the development and optimization of strategies to control mosquitoes and mosquito-borne diseases. Problems with the morphological identification of mosquito species have led to the use of molecular identification techniques, in particular the Folmer cytochrome c oxidase subunit I (COI) PCR system (FCOS), originally designed to identify a range of other invertebrates. As there can be difficulties identifying mosquitoes using FCOS, we re-evaluated the FCOS primers and developed a new COI-based SYBR PCR (the Auburn COI system—AUCOS) to improve the molecular identification of mosquitoes. Sequence data in GenBank for 33 species from 10 genera of mosquitoes were used to develop our AUCOS primers. Two molecular assays (AUCOS, FCOS) and morphological identification were carried out on mosquitoes collected from the field in Auburn, Alabama (USA) and on Saint Kitts. With a convenience sample of individual mosquitoes comprising 19 ....

United States , Baden Wüberg , Auburn University , National Center , Johnw Hock Company , Informax Inc , Rancho Dominguez , New Standard Miniature , Saint Kitts , Vernon Hills , Pipiens Assemblage , Bertin Instruments , Thigh Pure , Preparation Kit , Roche Diagnostics , North Bethesda , Roche Diagnostics Gmbh , Platinum Taq , Biotechnology Information ,

The Centres for Disease Control light trap (CDC-LT) and the human decoy trap (HDT) compared to the human landing catch (HLC) for measuring Anopheles biting in rural Tanzania | Malaria Journal

Vector mosquito biting intensity is an important measure to understand malaria transmission. Human landing catch (HLC) is an effective but labour-intensive, expensive, and potentially hazardous entomological surveillance tool. The Centres for Disease Control light trap (CDC-LT) and the human decoy trap (HDT) are exposure-free alternatives. This study compared the CDC-LT and HDT against HLC for measuring Anopheles biting in rural Tanzania and assessed their suitability as HLC proxies. Indoor mosquito surveys using HLC and CDC-LT and outdoor surveys using HLC and HDT were conducted in 2017 and in 2019 in Ulanga, Tanzania in 19 villages, with one trap/house/night. Species composition, sporozoite rates and density/trap/night were compared. Aggregating the data by village and month, the Bland–Altman approach was used to assess agreement between trap types. Overall, 66,807 Anopheles funestus and 14,606 Anopheles arabiensis adult females were caught with 6,013 CDC-LT, 339 indoor-HLC, 13 ....

Burkina Faso , Johnw Hock Company , World Health Organization , Measuring Anopheles , Disease Control ,

Early Release - Anopheles stephensi Mosquitoes as Vectors of Plasmodium vivax and falciparum, Horn of Africa, 2019 - Volume 27, Number 2—February 2021 - Emerging Infectious Diseases journal

, Temesgen Ashine
1, Hiwot Teka, Endashaw Esayas, Louisa A. Messenger, Wakweya Chali, Lisette Meerstein-Kessel, Thomas Walker, Sinknesh Wolde Behaksra, Kjerstin Lanke, Roel Heutink, Claire L. Jeffries, Daniel Abebe Mekonnen, Elifaged Hailemeskel, Surafel K. Tebeje, Temesgen Tafesse, Abrham Gashaw, Tizita Tsegaye, Tadele Emiru, Kigozi Simon, Eyuel Asemahegn Bogale, Gedeon Yohannes, Soriya Kedir, Girma Shumie, Senya Asfer Sabir, Peter Mumba, Dereje Dengela, Jan H. Kolaczinski, Anne Wilson, Thomas S. Churcher, Sheleme Chibsa, Matthew Murphy, Meshesha Balkew, Seth Irish, Chris Drakeley, Endalamaw Gadisa, and Teun Bousema
Author affiliations: Armauer Hansen Research Institute, Addis Ababa, Ethiopia (F.G. Tadesse, T. Ashine, H. Teka, E. Esayas, W. Chali, S.W. Behaksra, D.A. Mekonnen, E. Hailemeskel, S.K. Tebeje, T. Tafesse, A. Gashaw, T. Tsegaye, T. Emiru, E.A. Bogale, G. Shumie, S.A. Sabir, E. Gadisa); Radboud University Medical Center, Nijmegen, the Netherl ....

Graphpad Software Inc , Johnw Hock Company , Awash Sebat Kilo , Graphpad Prism , Arabian Peninsula , Southeast Asia , Anopheles Stephensi , Horn Of Africa , Membrane Feeding , Plasmodium Falciparum , Plasmodium Vivax , Urban Malaria , Vector Competence , அரேபியன் தீபகற்பம் , தென்கிழக்கு ஆசியா , வளர்ந்து வருகிறது , சவ்வு உணவளித்தல் , பரவும் முறை , நகர்ப்புற மலேரியா , திசையன் திறன் ,