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Georgia DNR Investigating Trout Diseases at Hatcheries

Georgia DNR Investigating Trout Diseases at Hatcheries
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Using Dynamic Light Scattering (DLS) to Analyze Virus Particles


Using Dynamic Light Scattering (DLS) to Analyze Virus Particles
Conventional visual techniques of detecting virus particles in solution provide a glimpse of an extremely small sampling volume, while the method of particle analysis employing dynamic light scattering generates an ensemble
average of particles inside the solutions.
Virus particles incubated in the laboratory pose an issue, as they need to be grown inside cells in a media containing albumin and additional small proteins, like those inside Fetal Bovine Serum (FBS) or Minimum Essential Media (MEM) solutions.
When virus particles are emitted from the cells, the cell pieces are big and can be divided by centrifugation, however the lesser proteins of the media cannot be eliminated. Cautious choice of the distribution parameters from a dynamic light scattering experiment permits clear observation of the size distributions of virus particles in the presence of significantly smaller proteins that make up the ....

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Using Dynamic Light Scattering (DLS) to Analyze Virus Particles


Using Dynamic Light Scattering (DLS) to Analyze Virus Particles
Conventional visual techniques of detecting virus particles in solution provide a glimpse of an extremely small sampling volume, while the method of particle analysis employing dynamic light scattering generates an ensemble
average of particles inside the solutions.
Virus particles incubated in the laboratory pose an issue, as they need to be grown inside cells in a media containing albumin and additional small proteins, like those inside Fetal Bovine Serum (FBS) or Minimum Essential Media (MEM) solutions.
When virus particles are emitted from the cells, the cell pieces are big and can be divided by centrifugation, however the lesser proteins of the media cannot be eliminated. Cautious choice of the distribution parameters from a dynamic light scattering experiment permits clear observation of the size distributions of virus particles in the presence of significantly smaller proteins t ....

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