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ddPCR: A powerful tool for cell therapy studies

Discover how the use of ddPCR technology allows assays to be run with a single surrogate calibration curve ....

Application Note , Gene Therapy , Droplet Digital Pcr ,

"Application of digital PCR for public health-related water quality mon" by Ananda Tiwari, Warish Ahmed et al.

Digital polymerase chain reaction (dPCR) is emerging as a reliable platform for quantifying microorganisms in the field of water microbiology. This paper reviews the fundamental principles of dPCR and its application for health-related water microbiology. The relevant literature indicates increasing adoption of dPCR for measuring fecal indicator bacteria, microbial source tracking marker genes, and pathogens in various aquatic environments. The adoption of dPCR has accelerated recently due to increasing use for wastewater surveillance of Severe Acute Respiratory Coronavirus 2 (SARS-CoV-2) - the virus that causes Coronavirus Disease 2019 (COVID-19). The collective experience in the scientific literature indicates that well-optimized dPCR assays can quantify genetic material from microorganisms without the need for a calibration curve and often with superior analytical performance (i.e., greater sensitivity, precision, and reproducibility) than quantitative polymerase chain reaction (qPC ....

Severe Acute Respiratory Coronavirus , Coronavirus Disease , Digital Pcr , Droplet Digital Pcr , Public Health Microbiology , Ater Microbiology ,

Transitioning from quantitative PCR to droplet digital PCR

Bio-Rad discusses migrating assays from real-time quantitative PCR (qPCR) to Droplet Digital PCR (ddPCR) ....

Application Note , Droplet Digital , Quantitative Pcr , Droplet Digital Pcr , Molecular Biology , Cell And Gene Therapy ,