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Central and peripheral clock synchronization vital for optimal tissue function

Discovered in the 1970s, circadian clocks are essential for the regulation of biological time in most cells in the human body. ....

United States , San Antonio , Comunidad Autonoma De Cataluna , El Salvador , San Diego , University Of Texas , Patrick Simon Welz , Thomas Mortimer , Mireia Vaca Dempere , Antonio Serrano , Eusebio Perdiguero , Aznar Benitah , Barun Kumar , Life Sciences , Institute For Research , European Research Council , Pompeu Fabra University , Ministry Of Science , Caixa Foundation , Lilliane Bettencourt Foundation , Novo Nordisk Foundation , University Of California , Hospital Del Mar Research Institute , Altos Labs San Diego Institute Of Science , University Of Texas Health , Humanitas University ,

Genetic studies shed light on hypertension risk but fall short of revealing new treatments

Genetic variant research in hypertension has identified numerous associations but is unlikely to yield new treatments, emphasizing the gap between scientific discovery and clinical application. ....

David Curtis , University College London , Genetics Institute , Genetic Variants Associated , Hypertension Risk , Image Credit , Shine Nucha , United Kingdom , Exome Chip , Blood Pressure , Dna Replication ,

AI tool helps develop the most complete map of the bacterial essential interactome

Universitat Autonoma de Barcelona (UAB) researchers have produced the most complete map of the bacterial essential interactome, i.e. how proteins combine and interact to perform functions essential for their survival. ....

Comunidad Autonoma De Cataluna , Department Of Biochemistry , Autonoma De Barcelona , Molecular Biology , Universitat Aut , Marc Torrent Burgas , Artificial Intelligence , Cell Division , Cell Membrane , Dna Replication , Molecular Biology ,

"Single-Molecule Fluorescence Imaging of DNA Replication Stalling at Si" by Kelsey S. Whinn, Nischal Sharma et al.

DNA replication in cells occurs on crowded and often damaged template DNA, forming potentially deleterious roadblocks to the progressing replication fork. Numerous tools have been developed to investigate the mechanisms of DNA replication and the fate of stalled replication forks. Here, we describe single-molecule fluorescence imaging methods to visualize processive DNA replication and replication fork stalling at site-specific nucleoprotein complexes. Using dCas9 as a protein barrier and rolling-circle DNA templates, we visualize effective, stable, and site-specific blocking of the Escherichia coli replisome. Additionally, we present a protocol to produce an 18-kb rolling-circle DNA template that provides increased spatial resolution in imaging the interplay between replisomes and roadblocks. These methods can be used to investigate encounters of the replisome with nucleoprotein complexes at the single-molecule level, providing important mechanistic details of replisome stalling and d ....

Dna Replication , Olling Circle Dna , Ingle Molecule Imaging , Talled Replication ,

Unearthing individual-level mutation risk patterns

Mutations, which occur continuously in every cell of our bodies, are a key contributor to cancer, ageing, and neurodegeneration. ....

Comunidad Autonoma De Cataluna , Marina Salvadores , Fran Supek , Cienciae Innovaci , Institute For Research , Genome Data Science , Spanish Ministerio De Ciencia , Cell Proliferation , Dna Replication ,