Developing Hwanglyeonhaedok-tang-mediated biosynthesized gold nanoparticles and investigating their immune-enhancing properties in macrophages and splenocytes.
A) Representative fluorescent images of SGNs with different treatments. (
B) Average neurite length. p 0.001, p 0.0001 by one-way ANOVA. (
C) The neurite orientation index (cosθ). Cos ~1 for the neurites along the direction of magnetic force. p 0.05 by one-way ANOVA.
SPIO and SPIO+MF Promote the Development of Growth Cones
We further determined the effect of the SPIO nanoparticles and the MF on SGN growth cones and synaptic density by immunofluorescence. We stained the cultured SGNs with the neuron marker anti-β tubulin and stained the actin-supported growth cone with phalloidin at DIV3. The growth cone area of the neurites was larger, and the average filopodia length was longer when treated with SPIO or SPIO+MF compared to controls (Figure 5A, B and D). When exposed to the MF only, the growth cone area, the number of filopodia, and the average filopodia length were not significantly different compared to controls (p 0.05, Figure 5B–D). The number of filopod