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Background: Diabetes mellitus is a heterogeneous clinical syndrome secondary to defects in insulin secretion, action, or both. The worldwide fatality and disability rate imposed diabetic patients for insulin self-administration regardless of a scarcity of their knowledge, attitude, and practices. Thus, the study aimed to assess knowledge, attitude, and practice towards insulin-self administration among diabetic patients attending Bedele Hospital, southwest of Ethiopia. Methods: A facility-based cross-sectional study was conducted by a consecutive sampling technique at Bedele Hospital from 15 December 2019 to 22 January 2020. A pretested structured English version relevant questionnaire translated to local language was administered on 196 subjects. Data were tested for clarity, consistency and analyzed using The Statistical Package for Social Sciences version 23 and summarized using descriptive statistics in the form of tables and figures. ....