G,
H) for both HT29DDP and HCT116-LOHP cells. The graphs of b, d, f. and h are the densitometry results of corresponding WB assays.
Notes: P 0.05; P 0.01.
Combination Treatment Promoted Apoptosis in Drug-Resistant Colon Cancer Cells
We further analyzed the effect of combinational therapy on apoptosis of colon cancer cells. The results (Figure 5A–D) for HT29DDP and HCT116-LOHP cells, respectively) showed that the apoptotic rates of different treatment groups were higher than those of the untreated control group; the difference between PTX-16nM group and the control group was statistically significant (P 0.05). The difference between combination therapy group (PTX-8nM + BEZ 50nM group and PTX-16nM + BEZ 50nM group) and the control group was also statistically significant (P 0.01). These data indicate that PTX combined with BEZ235 can more effectively promote apoptosis in drug-resistant colon cancer as compared with PTX alone. We then further analyzed the levels of Bcl-2 pr