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D), (
E) Hydrodynamic diameters and Zeta potentials of cAuNPs and mAuNPs measured with dynamic light scattering (DLS). Note: hydrodynamic diameter and Zeta potential measurements are presented in the supporting information.
Cellular Uptake of mAuNPs
Shown in Figure 2A are photographs taken under dark field microscope of B16-F10 cells cultured with and without mAuNPs for 24 h. Numerous small spots in B16-F10 cells co-cultured with mAuNPs for 24 h were visible compared to controls, indicating that mAuNPs entered B16-F10 cells. Next, we investigated the cellular uptake of mAuNPs after B16-F10 cells were co-cultured with mAuNPs at different concentrations by ICP-AES (Figure 2B). The amount of cellular uptake of mAuNPs increased with the AuNPs co-culture concentrations in the range of ~0–60 μg/mL. The cellular uptake amount reached 3.6 pg/cell at the co-culture concentration of 10 μg/mL. If the weight of 10 ....

United States , Wu Wei , Bax Bcl , Institute Of Modern Physics , National Institutes Of Health , Administration Of Aunps , Chinese Academy Of Sciences Shanghai , Beckman Coulter Co , Cell Bank , Thermo Electron Co , Jk Chemical Co , Imagej National Institutes Of Health , Ion Therapy Center , Precision Co Ltd , Wuhan Sanying Biotechnology Company , Supporting Information , Chinese Academy , Heavy Ion Therapy Center , Hydroxyl Radical , Reactive Oxidative , Tumor Bearing Mice , National Institutes , Animal Use Protocol , Modern Physics , Institutional Animal Care , Use Committee ,