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Researchers use single-cell RNA sequencing technologies to track cellular development in leaves
How do we become a complex, integrated multicellular organism from a single cell?
While developmental biologists have long researched this fundamental question, Stanford University biologist and HHMI investigator Dominique Bergmann s recent work on the plant Arabidopsis thaliana has uncovered surprising answers.
In a new study, published April 5 in
Developmental Cell, led by Bergmann and postdoctoral scholar Camila Lopez-Anido, researchers used single-cell RNA sequencing technologies to track genetic activity in nearly 20,000 cells as they formed the surface and inner parts of an Arabidopsis leaf.
Through this highly detailed technique, the researchers captured transient and rare cell states and found a surprising abundance of ambiguity in how cells traversed various identities, particularly early on within the stem cell population.
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IMAGE: A drawing series by artist Virginia Lopez-Anido that was inspired by research by her sister, Camila Lopez-Anido, a postdoctoral scholar at Stanford who studies cellular development in plants. view more
Credit: Virginia Lopez-Anido
How do we become a complex, integrated multicellular organism from a single cell?
While developmental biologists have long researched this fundamental question, Stanford University biologist and HHMI investigator Dominique Bergmann s recent work on the plant Arabidopsis thaliana has uncovered surprising answers.
In a new study, published April 5 in
Developmental Cell, led by Bergmann and postdoctoral scholar Camila Lopez-Anido, researchers used single-cell RNA sequencing technologies to track genetic activity in nearly 20,000 cells as they formed the surface and inner parts of an Arabidopsis leaf. Through this highly detailed technique, the researchers captured transient and rare cell states and found a surprising abundance