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2Laboratory for Pharmaceutical Biotechnology, University of Ghent, Ghent, Belgium 3ProGenTomics, Ghent, Belgium 4Laboratory of Proteinscience, Proteomics and Epigenetic Signaling, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium 5Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium Chikungunya virus (CHIKV) is an arbovirus with a global spread and significant public health impact. It is a positive stranded RNA alphavirus belonging to the Togaviridae family. However, many questions about the replication cycle of CHIKV remain unanswered. The entry process of CHIKV is not completely understood nor are the associated virus-receptor interactions fully identified. Here, we designed an affinity purification mass spectrometry coupled approach that allowed the identification of factors that facilitate entry of CHIKV in human cells. The identified entry factors were further validated using CRISPR/Cas9. In HEK293T

United statesSouth africaProvence alpes côd azurBasse normandieKrung thep mahanakhonUbon ratchathaniHaute normandieSouth africanMohamed hussainHela matusaliIndian oceanTatsuo shioda mahidolFeng zhangInstitute of technologyAndres merits university of tartuKosaka center