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Chromatography: Monitoring Monoclonal Antibody Breakthrough Curves

Sponsored by tec5USA IncMar 1 2021 Raman spectroscopy is a non-destructive analysis technique that is outstanding for measuring the frequencies of chemical bond vibrations. As these occur at particular energies relying on the exact chemical environment around the bond, a survey of varying frequencies can be used to ‘fingerprint’ distinct chemical compounds, even for complex chemical species, including monoclonal antibodies. Raman spectroscopy can be utilized to quantify the levels of impurities for confirmation of the production of the desired products. One of the major challenges in the preparation of monoclonal antibodies is usually when chromatography is used in the purification process.

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