Confirmation of Gene Mutation
For all 12
H. pylori strains used, the sequence of 16SrDNA, a ribosomal gene involved in protein production, was analyzed, and the presence or absence of gene mutation was examined. Table 3 shows the number of base substitutions and the number of deletions/insertions in the 16SrDNA sequence. Of these, susceptible strain 3 (S3), amoxicillin-resistant strain 1 (AC1), amoxicillin-resistant strain 2 (AC2), clarithromycin-resistant strain 1 (CH1), and metronidazole-resistant strain 2 (MZ2) were resistant to disulfiram in the growth suppression test. Strains resistant to disulfiram tended to have fewer base deletions/insertions.
Table 3 16S rDNA Sequence
Discussion
Eradication therapy using amoxicillin and clarithromycin is performed for