Swiss-Romanian Chamber of Commerce Hosts Successful Conference on the Swiss Model in Family Businesses, Inspiring Romanian Entrepreneurs nineoclock.ro - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from nineoclock.ro Daily Mail and Mail on Sunday newspapers.
Industrial application of icariin and baohuoside I has been hindered by the short supply to a great extent. In this work, a novel GH78 α-l-rhamnosidase AmRha catalyzed the bioconversion of low-value epimedin C in crude Epimedium Folium flavonoids (EFs) to icariin and baohuoside I was developed. Firstly, the high-level expression of AmRha in Komagataella phaffii GS115 attained an enzyme activity of 571.04 U/mL. The purified recombinant AmRha could hydrolyze α-1,2-rhamnoside bond between two rhamnoses (α-Rha(2 → 1)α-Rha) in epimedin C to produce icariin with a molar conversion rate of 92.3%, in vitro. Furtherly, the biotransformation of epimedin C to icariin by the recombinant Komagataella phaffii GS115 cells was also investigated, which elevated the EFs concentration by fivefold. In addition, biotransformation of epimedins A-C and icariin in the raw EFs to baohuoside I was fulfilled by a collaboration of AmRha and β-glucosidase/β-xylosidas
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This season's Champions League group stage draw takes place today with 32 European teams set to learn their fate. The draw is due to start at 5pm (BST) which is 12pm (ET) or 9am (PT). Background readingChampions League group stage draw: How to watch, what time and where is it located?UEFA Champions League 2022-23 explained: New rules and law changes in World Cup yearWhy is this the smallest Champions League ever? Russian and Belarusian ban explainedFollow the draw live right here on The Athletic.