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Cell envelope and stress-responsive pathways underlie an evolved oleaginous Rhodotorula toruloides strain multi-stress tolerance | Biotechnology for Biofuels and Bioproducts

The red oleaginous yeast Rhodotorula toruloides is a promising cell factory to produce microbial oils and carotenoids from lignocellulosic hydrolysates (LCH). A multi-stress tolerant strain towards four major inhibitory compounds present in LCH and methanol, was derived in our laboratory from strain IST536 (PYCC 5615) through adaptive laboratory evolution (ALE) under methanol and high glycerol selective pressure. Comparative genomic analysis suggested the reduction of the original strain ploidy from triploid to diploid, the occurrence of 21,489 mutations, and 242 genes displaying copy number variants in the evolved strain. Transcriptomic analysis identified 634 genes with altered transcript levels (465 up, 178 down) in the multi-stress tolerant strain. Genes associated with cell surface biogenesis, integrity, and remodelling and involved in stress-responsive pathways exhibit the most substantial alterations at the genome and transcriptome levels. Guided by the suggested stress response

Atpase-neo
Genome-institute
Additional-file
Supplementary-table
Differentially-expressed-genes
Joint-genome-institute
Eukaryotic-orthologous-groups
Functional-enrichment-analysis

CAHG Culture and Lexicon. : languagehat.com

CAHG Culture and Lexicon. : languagehat.com
languagehat.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from languagehat.com Daily Mail and Mail on Sunday newspapers.

Central-african-republic
Babongo
00
Central-african
Baka-ubangian
Bogdan-pricop
Javier-blanco-portillo
Lucio-vinicius
Andrea-bamberg-migliano
Balthasar-bickel
Cecilia-padilla-iglesias
Dmitry-pruss

Mapping model units to visual neurons reveals population code for social behaviour

The rich variety of behaviours observed in animals arises through the interplay between sensory processing and motor control. To understand these sensorimotor transformations, it is useful to build models that predict not only neural responses to sensory input1–5 but also how each neuron causally contributes to behaviour6,7. Here we demonstrate a novel modelling approach to identify a one-to-one mapping between internal units in a deep neural network and real neurons by predicting the behavioural changes that arise from systematic perturbations of more than a dozen neuronal cell types. A key ingredient that we introduce is ‘knockout training’, which involves perturbing the network during training to match the perturbations of the real neurons during behavioural experiments. We apply this approach to model the sensorimotor transformations of Drosophila melanogaster males during a complex, visually guided social behaviour8–11. The visual projection neurons at the

Bloomington-stock-center
Pearson
Supplementary-table
Bloomington-stock
Extended-data
Supplementary-videos
Supplementary-video
Nature-portfolio-reporting-summary

Preventable Premature Deaths from the Five Leading Causes of Death in Nonmetropolitan and Metropolitan Counties, United States, 2010–2022

The Morbidity and Mortality Weekly Report (MMWR) Series is prepared by the Centers for Disease Control and Prevention (CDC).

Wyoming
United-states
Alaska
Washington
District-of-columbia
Georgia
Atlanta
Hyattsville
Maryland
American
Linda-schieb
Kevin-matthews

Reporting software and package versions is becoming more important than ever before

Reporting software and package versions is becoming more important than ever before
bmj.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from bmj.com Daily Mail and Mail on Sunday newspapers.

Supplementary-table