Post-translational modifications (PTMs) provide a rapid mechanism that enables protein phenotypic diversity so that proteins can react to external and internal disturbances and regulate cellular activity. This Biotech Brief discusses tools and methods that are employed in detecting these sub-stoichiometric and elusive protein modifiers, including mass spectrometry, antibody, artificial intelligence, ubiquitin-clipping, and specific uncaging-assisted biotinylation-based approaches. The article also discusses challenges in detecting PTMs, such as the development of PTM antibodies with high specificity and binding affinity.
Post-translational modifications (PTMs) provide a rapid mechanism that enables protein phenotypic diversity so that proteins can react to external and internal disturbances and regulate cellular activity. This Biotech Brief discusses tools and methods that are employed in detecting these sub-stoichiometric and elusive protein modifiers, including mass spectrometry, antibody, artificial intelligence, ubiquitin-clipping, and specific uncaging-assisted biotinylation-based approaches. The article also discusses challenges in detecting PTMs, such as the development of PTM antibodies with high specificity and binding affinity.
AlphaFold AI Uncovers New 3D Proteins with Open Data miragenews.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from miragenews.com Daily Mail and Mail on Sunday newspapers.