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IMAGE: The E. coli chromosome was split into three 1-Mb chromosomes, and the chromosome was used for genome implantation. view more
Credit: Rikkyo University
Escherichia coli (
E. coli) genome, consisting of 4.6 million base pairs of a single circular DNA, is too large to manipulate following the extraction and transfer to other bacteria.
In the present study, a group of Rikkyo University researchers led by Assistant Professor Takahito Mukai and Professor Masayuki Su etsugu has succeeded in splitting the E.coli genome into tripartite-genome of 1 million base pairs per genome (split-genome) using the smallest
E. coli genome strain established so far. In addition, they successfully extracted the split-genome from bacteria and installed it in other