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Image Credit: Likoper/Shutterstock.com L’occhio Della Mosca ( The Fly’s Eye) by Giambattista Odierna in 1644 CE. In that sensational book, the Italian naturalist provided the first detailed account of organic tissue – enabled by studying organic matter under the lens of newly invented microscopes. Now, the latest optical microscope technology is capable of imaging viruses that live in organisms. Conventional Light Microscopes: Widefield and Confocal Conventional light microscopes – widefield and confocal optical microscopes – can accurately depict image samples at resolutions of around 230 nm (widefield) or 180 nm (confocal) in the XY axes. On the Z-axis (analogous to focal length in photography), these traditional microscopy methods work at resolutions of around 1000 nm (widefield) or 500 nm (confocal).

Frits zernikeGiambattista odiernaLikoper shutterstockGabriella ficzBen pilkingtonGeorges nomarskiMichaelw davidsonL occhio della moscaLight microscopesTheoretical resolutions limitsResearchers use light microscopesStudy virusesFluorescence microscopyCryo electron tomographyTransfected mammalian cellsResolution limit