Latest Breaking News On - Post response - Page 1 : comparemela.com

Invasive infections due to group A Streptococcus (GAS) advance rapidly causing tissue degradation and unregulated inflammation. Neutrophils are the primary immune cells that respond to GAS infection, where it is known that the degree of the neutrophil inflammatory response can influence the outcome of infection. The response of human neutrophils to GAS has not been described in depth. This thesis aimed to characterise the neutrophil response to GAS using in vitro and in vivo models of invasive infection, to further understand the contribution of neutrophils to invasive GAS disease. The current project optimised methodology to investigate the presence of intracellular neutrophil proteins via immunoblot, including caspases, where increased proteases and phosphatase inhibitors decreased protein degradation in neutrophil lysates. Methods of immunoblot normalisation were assessed and revealed total protein is the most appropriate method when undertaking densitometric quantification of neutr

Post responseInnate immunity