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Artificial sweeteners linked to marked effects on small intestinal microbiome

CHICAGO — Greater consumption of artificial sweeteners, specifically those that do not contain aspartame, resulted in “marked effects” on the duodenal microbiome profile, according to data presented at Digestive Disease Week. “As physicians, we see a number of patients who consume artificial sweeteners for a variety of reasons,” Ruchi Mathur, MD, director of clinical

Soil environmental DNA metabarcoding in low-biomass regions requires p by Pamela Olmedo-Rojas, Gert Jan Jeunen et al

Environmental DNA is a powerful tool for monitoring biodiversity. Although environmental DNA surveys have successfully been implemented in various environments, protocol choice has been shown to affect results and inferences. Thus far, few method comparison studies for soil have been undertaken. Here, we optimized the workflow for soil metabarcoding through a comparative study encompassing variation in sampling strategy (individual and combined samples), DNA extraction (PowerSoil®, NucleoSpin® Soil, PowerSoil® + phosphate buffer and NucleoSpin® Soil + phosphate buffer) and library preparation (one-step and two-step quantitative polymerase chain reaction methods). Using a partial 18S rRNA marker, a total of 309 eukaryotic taxa across 21 phyla were identified from Antarctic soil from one site in the Larsemann Hills. Our optimized workflow was effective with no notable reduction in data quality for a considerable increase in time and cost efficiency. The NucleoSpin® Soil + phosphate

expert reaction to study looking at birth method, and microbiome and response to vaccinations in children

expert reaction to study looking at birth method, and microbiome and response to vaccinations in children
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MaaT Pharma Confirms Positive Results from Completed Phase 1b CIMON Study Evaluating MaaT033 in Blood Cancer Patients

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