http://www.mircode.org/) were used to identify the potential binding sequences of miR-140-5p and LINC00504. SKBR3 and MDA-MB-231 cells were seeded in 24-well plates. The cells were cotransfected with miR-140-5p or microRNA NC and luciferase reporter constructs containing WT-LIC00504 or Mut-LINC00504, as previously described.
13 The luciferase activity was detected using a Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime Institute of Biotechnology, Shanghai, China), according to the manufacturer’s protocol. Mutations in the putative binding sites of LINC00504 were made using a Quick-change site-directed mutagenesis kit (200518, Agilent Technologies, Santa Clara, USA). The primers were as follows: LINC00504, Forward primer: 5ʹ-GTGACTCGAGCTTGCCTCTGCCATGT-3ʹ, Reverse primer: 5ʹ-GTGAGCGGCCGCTTTCAGAGTGAAACAATACTT-3ʹ; Mut-LINC00504, Forward primer: 5`-GGATTACAGGCGTGAGGGTGAC-3`; and Reverse primer: 5`-GTCACCCTCACGCCTGTAATCC-3`.
Building better bubbles for ultrasound could enhance image quality, facilitate treatments
Building better bubbles for ultrasound could enhance image quality, facilitate treatments
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Karen R. Olsen
Ultrasound is a non-invasive technique that uses sound waves to either generate images of tissues inside of the body, or to interact with tissues as a therapeutic tool – to break up gallstones, increase blood flow, or ablate tumors, for instance. Ultrasound contrast agents, which are typically tiny bubbles filled with gas, can enhance the reflection of ultrasound waves to improve the quality of an ultrasound image. However, commercially available contrast agents are confined to the blood vessels, typically remain in the bloodstream for less than 10 minutes, and are used in only a handful of settings in the United States.
Global Molecular Probes Market | Application ,Regional Analysis & Top key Players like BioRad Laboratories, Hologic, Sysmex Corporation & Dako
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Molecular Probes Market analysis report published on Qualiket Research website which includes analysis of market forecast, share, size and dynamics covered in the report. This is latest report comprises overview of the Molecular Probes industry, with an informative explanation. The Molecular Probes market has been studied in detail to arrive an accurate & insightful conclusion regarding the current market trends noted in the industry.
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Molecular probes are the part of small RNA or DNA which recognizes the complementary sequences in DNA and RNA molecules. Molecular probes are used in identification and isolation of specific RNA or DNA sequences from organism. Molecular probes offers as the resources for various applications such as chromosomal mapping, molecular cytogenetics, and DNA fin
Global Molecular Probes Market | Application ,Regional Analysis & Top key Players like BioRad Laboratories, Hologic, Sysmex Corporation & Dako
iCrowd Newswire
Molecular Probes Market analysis report published on Qualiket Research website which includes analysis of market forecast, share, size and dynamics covered in the report. This is latest report comprises overview of the Molecular Probes industry, with an informative explanation. The Molecular Probes market has been studied in detail to arrive an accurate & insightful conclusion regarding the current market trends noted in the industry.
Get Free Sample Copy of this Report @
Molecular probes are the part of small RNA or DNA which recognizes the complementary sequences in DNA and RNA molecules. Molecular probes are used in identification and isolation of specific RNA or DNA sequences from organism. Molecular probes offers as the resources for various applications such as chromosomal mapping, molecular cytogenetics, and DNA fin
Search jobs Cambridge Research Biochemicals signs distribution deal with Fluoroprobes, LLC
Cleveland, UK, 29th April 2021 / Sciad Newswire / Leading UK peptide and antibody producers, Cambridge Research Biochemicals (CRB) have today signed a new distribution agreement with Fluoroprobes, LLC (FPL) and Click Chemistry Tools (CCT) to add FPL and CCT’s generic fluorescent activated dyes and click chemistry enabled click-reactive dyes to their existing service portfolio. The distribution agreement grants CRB the rights to market and sell the generic and improved Alexa Fluor-based DISCOVERY® Dyes both as part of their custom services, and with their catalogue peptide (DISCOVERY® Peptides) and antibody (DISCOVERY® Antibodies) services in the UK and Europe.