Scientific background
The 2019-nCoV Neutralization ELISA Kit from SignalChem is an enzyme-linked immunosorbent assay meant for detecting antibodies or other molecular proteins that prevent the binding of human ACE2 and SARS-CoV-2 S1 protein in serum or other biological specimens.
Built on indirect ELISA principles, the 2019-nCoV Neutralization ELISA Kit uses recombinant human ACE2 protein coated on a 96-well plate.
Both controls and samples (with the required dilution) are blended with the 2019-nCOV S1 protein RBD-HRP and subsequently pipetted into the wells. The target neutralizing proteins or antibodies in the samples and controls will fully or partly block the binding of the S1 protein RBD-HRP to the immobilized ACE2 protein.
The 2019-nCoV High Sensitivity S1 Protein ELISA Kit v2.0 available from SignalChem is a sandwich enzyme-linked immunosorbent assay that can be used for the quantitative measurement of 2019-nCoV Coronavirus Spike protein.
The S1 Protein ELISA uses a capture anti-S1 protein antibody coated on a 96-well plate. Samples and calibrators are pipetted into the wells, and the target protein contained in the samples and calibrators attaches to the immobilized capture antibody.
The HRP-labeled Detection antibody is added after washing the wells. Once the unbound HRP-antibodies are washed away, a colorimetric substrate solution (TMB) is pipetted into the wells. The absorbance of the color at 450 nm is quantified. The higher the intensity of color development within the wells, the more the amount of target protein bound.
The recombinant Llama VHH-Human IgG Antibody from SignalChem Biotech is a high-sensitivity SARS-CoV-2 spike protein detection assay. The particular activity was quantified using a couple of Functional ELISA systems, and this was done by testing the binding ability of the nanobody to SARS-CoV-2 spike protein.
Scientific background
SARS-CoV-2 the severe acute respiratory syndrome-related novel coronavirus has been responsible for causing the pandemic of respiratory diseases (COVID-19) throughout the world in 2020.
1
The spike glycoprotein (S) of coronavirus is part of the type I transmembrane protein that contains two subunits, S1 and S2.
2 These subunits are also known to be the main components to attach to host cells via the interaction with angiotensin-converting enzyme 2 (ACE2).