Mark Pelley, who labels himself the Snake Hunter, was chasing the serpent at a house in the outer northern Melbourne suburb of Doreen about 6.45pm on Monday, when it made a surprise attack.
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Mr Pelley said the snake was likely seeking shelter from the hot weather.
He said it was the first time he had been called to remove a snake from beneath a microwave in all his years working across Australia.
Mr Pelley told The Australian that the snake had enough venom to kill all of the six staff members present in the office.
Melbourne s best known snake catcher, Mark Pelley, caught the snake
The eastern tiger is considered the fifth most deadly snake in the world, with venom strong enough to kill a victim within an hour of a bite. They leave you alone if you leave them alone, but if you try to pick them up or kill them they ll bite you, Mr Pelley said.