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2Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil 3Faculty of Medical Sciences, University of Campinas, Campinas, Brazil 4Laboratory of Digital Signal Processing in Real Time, School of Electrical and Computer Engineering, University of Campinas, Campinas, Brazil 5Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, United States Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to pain, discomfort, and unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app for dose calculation and a standard dose calculation. A total of 939 C57BL/6J and Swiss mice were analyzed. We collected data on

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