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Preserving the Valley s water heritage

Five months on and KUKL still testing water supply pipeline of Melamchi

KATHMANDU, May 16: Due to the managerial weakness of Kathmandu Upatyaka Khanepani (KUKL), the state-owned utility is losing revenue worth millions due to failure in billing at the customers' houses for distributing water from Melamchi through a new pipeline.

Frontiers | Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal

Antibiotic resistant bacteria-associated infections are responsible for more than 1.2 million annual deaths worldwide. In low- and middle-income countries (LMICs), the consumption of antibiotics for human and veterinary uses is not regulated effectively. Overused and misused antibiotics can end up in aquatic environments, which may act as a conduit for antibiotic resistance dissemination. However, data on the prevalence of antibiotic resistance determinants in aquatic environments are still limited for LMICs. In this study, we evaluated the prevalence and concentration of antibiotic resistance genes in different drinking and environmental water sources collected from the Kathmandu Valley, Nepal using droplet digital PCR to understand the current situation of ARG contamination. River water and shallow dug well water were the most contaminated with ARGs. Almost all samples contained sul1 (95%), and intI1 and tet(A) were detected in 83% and 60% of the samples, respectively. Maximum ARG co

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