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Supersize the cell: Unlocking genome secrets through expansion microscopy

Supersize the cell: Unlocking genome secrets through expansion microscopy
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Discovery may open novel avenues to prevent or treat hematological malignancies

Scientists at Yale Cancer Center have discovered new consequences of specific gene mutations that play a role in the development of myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML).

STED microscopy reveals sub-cellular details in living mice

STED microscopy reveals sub-cellular details in living mice 06 Apr 2021 Yale project achieves super-resolution 3D imaging in native tissue environment. Spot the diference: 3D STED A group at Yale University has successfully captured 3D super-resolution images from the brains of living mice. As published in Optica, the breakthrough was based on the use of stimulated emission depletion (STED) microscopy, one of the family of techniques developed to allow imaging beyond the normal diffraction limit of optical microscopy. The principles behind STED, for which Stefan Hell shared the 2014 Nobel prize for chemistry, involve the addition of a quenching laser pulse to conventional fluorescence excitation, one delivered in a ring-shaped profile. Resolution at the central hole of this pulse can be driven down below the diffraction limit under certain conditions.

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