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IJMS | Free Full-Text | Myogenetic Oligodeoxynucleotide Induces Myocardial Differentiation of Murine Pluripotent Stem Cells

An 18-base myogenetic oligodeoxynucleotide (myoDN), iSN04, acts as an anti-nucleolin aptamer and induces myogenic differentiation of skeletal muscle myoblasts. This study investigated the effect of iSN04 on murine embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). In the undifferentiated state, iSN04 inhibited the proliferation of ESCs and iPSCs but did not affect the expression of pluripotent markers. In the differentiating condition, iSN04 treatment of ESCs/iPSCs from day 5 onward dramatically induced differentiation into Nkx2-5+ beating cardiomyocytes with upregulation of Gata4, Isl1, and Nkx2-5, whereas iSN04 treatment from earlier stages completely inhibited cardiomyogenesis. RNA sequencing revealed that iSN04 treatment from day 5 onward contributes to the generation of cardiac progenitors by modulating the Wnt signaling pathway. Immunostaining showed that iSN04 suppressed the cytoplasmic translocation of nucleolin and restricted it to the nucleoli. These resu

United kingdomUnited statesBaden wübergSan diegoJackson immunoresearchWayne rasbandNational bioNational institute of healthMyocardial differentiationMesoderm differentiationWnt signaling pathwayNucleolin translocationNot affect myocardial cellFujifilm wako chemicalsRegenerative medicineNational bio resource project

mRNA vaccine quality analysis using RNA sequencing | Nature Communications

The success of mRNA vaccines has been realised, in part, by advances in manufacturing that enabled billions of doses to be produced at sufficient quality and safety. However, mRNA vaccines must be rigorously analysed to measure their integrity and detect contaminants that reduce their effectiveness and induce side-effects. Currently, mRNA vaccines and therapies are analysed using a range of time-consuming and costly methods. Here we describe a streamlined method to analyse mRNA vaccines and therapies using long-read nanopore sequencing. Compared to other industry-standard techniques, VAX-seq can comprehensively measure key mRNA vaccine quality attributes, including sequence, length, integrity, and purity. We also show how direct RNA sequencing can analyse mRNA chemistry, including the detection of nucleoside modifications. To support this approach, we provide supporting software to automatically report on mRNA and plasmid template quality and integrity. Given these advantages, we antic

Oxford nanoporeAgilent tapestationRna stranded library preparation kitIllumina truseqLibrary preparation kit

Cathepsin W restrains peripheral regulatory T cells for mucosal immune quiescence

Cathepsin W restrains peripheral regulatory T cells for mucosal immune quiescence
science.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from science.org Daily Mail and Mail on Sunday newspapers.

United statesQuintara biosciencesRane national institute of diabetesTransgenic mouse model laboratoryCell signaling technologyJackson laboratoryHistoserv incPham washington university school of medicineNational cancer instituteNational instituteKidney diseasesWashington university schoolGenome modificationGuide it longMouse modelInstitutional animal care

Zebrafish Rif1 impacts zygotic genome activation, replication timing, and sex determination

Zebrafish Rif1 impacts zygotic genome activation, replication timing, and sex determination
elifesciences.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from elifesciences.org Daily Mail and Mail on Sunday newspapers.

United statesBenjamini hochbergBiosciences facsaria iiiuPicard markduplicatesEscs liAndor zylaSanta claraSusannah rankin lafontBiosciences facscaliburDavid grunwald addgeneZymo researchGolden gate assemblyJackson immuno research laboratoriesNational institutes of health instituteOklahoma centerResearch computing core facilities

FKBP35 secures ribosome homeostasis in Plasmodium falciparum

FKBP35 secures ribosome homeostasis in Plasmodium falciparum
elifesciences.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from elifesciences.org Daily Mail and Mail on Sunday newspapers.

Ron dzikowskiVera mitesserMicrobeta filtermateMaisch gmbDavid fidockOrbitrap elicpse tribridPerkin elmerCorning incorporatedUniversity of glasgowWolfson merit royal society awardWellcome trust center awardSingapore ministry of educationProteomexchange consortiumSwiss national foundationBlood donation centerNonlinear dynamics