The effect of various ion pairing reagents (amines and acidic counterions) on the LC-UV and LC-MS analysis of oligonucleotides (ONs) has been studied. The type of amine and concentration of the counterion play a significant role in the retention and resolution of the ONs and strongly affect the MS data. In this application note, Agilent Technologies demonstrates that there are numerous variables available for optimization of the analytical conditions for ONs. The analyses were
Product News: Easy-to-use reversed-phase capillary columns and emitters improve high-resolution LC-MS analysis
25 Feb 2021
Proteomics, clinical peptides, and biopharmaceutical research laboratories performing high throughput, high-performance liquid chromatography (HPLC), or liquid chromatography-mass spectrometry (LC-MS) analysis can now benefit from novel capillary chromatography columns and nano- and capillary emitters, specifically designed for highly sensitive top-down, middle-down analysis.
These columns and emitters enable laboratories to achieve high-resolution, low-flow separations for intact proteins, monoclonal antibodies (mAbs) and peptides.
The Thermo Scientific MAbPac Reversed-Phase (RP) Capillary HPLC Column builds upon the separation power and reliability of the proven MAbPac RP chemistry in analytical flow. Together with the new Thermo Scientific EASY-Spray Nano and Capillary Emitters, the temperature-controlled MAbPac column enables maximum reliability and per