In drug development many applications are found for lipid-based nanoparticles (Lb-NPs) as successful transporters
for poorly water-soluble drugs and oligonucleotides in gene therapy.
Major advantages of lipids are their
high biocompatibility and biodegradability. In addition, advanced formulations using Lb-NP encapsulation of active ingredients can strongly contribute to targeted drug delivery in the body as well as stability and storage of the formulation.
The most important current medical applications include
cancer therapeutics and some of the current
COVID-19 vaccines.
Development and manufacturing of Lb-NP products often involves complex processes that need to be thoroughly understood and controlled to ensure optimum and constant quality of the medicine for patients. Therefore,
Background
Microalgae have attracted considerable interest due to their ability to produce a wide range of valuable compounds. Pulsed Electric Fields (PEF) has been demonstrated to effectively disrupt the microalgae cells and facilitate intracellular extraction. To increase the commercial viability of microalgae, the entire biomass should be exploited with different products extracted and valorized according to the biorefinery scheme. However, demonstrations of multiple component extraction in series are very limited in literature. This study aimed to develop an effective lipid extraction protocol from wet
Scenedesmus almeriensis after PEF-treatment with 1.5 MJ·kg
DW
−1. A cascade process, i.e., the valorization of several products in row, was tested with firstly the collection of the released carbohydrates in the water fraction, then protein enzymatic hydrolysis and finally lipid extraction. Biomass processed with high pressure homogenization (HPH) on parallel, served as be