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Reprinted with permission from Journal of Proteome Research © 2020 American Chemical Society (ACS) Mass spectrometry (MS) is a powerful method for biomarker analysis because it enables highly sensitive and accurate measurement of target molecules in clinical samples. The application of MS to clinical diagnosis, such as neonatal metabolic screening, has been progressing with a focus on metabolite markers. MS measurement of proteins is currently mainly used for novel marker discovery studies, but there is a growing interest in its application in clinical marker diagnosis as an alternative to immunoassays. MS-based quantification of protein biomarkers is mainly performed by a bottom-up approach using peptide fragments obtained by enzymatic protein digestion with trypsin. Standard digestion protocols require a reaction time of more than 20 hours, which is a rate-limiting factor in sample preparation workflows.

Kitasato universityHamamatsu university school of medicineEhime universityGel dissolutionResolved objective proteinsHamamatsu university schoolProteome researchAmerican chemical societyஹமாமட்சு பல்கலைக்கழகம் பள்ளி ஆஃப் மருந்துஎஹீமே பல்கலைக்கழகம்கேள் கலைப்புஹமாமட்சு பல்கலைக்கழகம் பள்ளிஅமெரிக்கன் இரசாயன சமூகம்