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LncRNA AFAP1-AS1 Modulates Gastric Cancer Cell Proliferation

13 and hepatocellular carcinoma. 14,15 In a previous analysis of the lncRNA expression profile using a microarray assay, we found that AFAP1-AS1 was upregulated in six GC tissues compared with paired non-cancerous tissues. To investigate the relationship between AFAP1-AS1 and GC, the expression of AFAP1-AS1 was further verified in a study involving a larger sample size composed of tissues and six different GC cell lines. In addition, the potential functions of AFAP1-AS1 on the proliferation, migration, and invasion of GC cells was also investigated in this study. Materials and Methods Tissue Samples and RNA Extraction In this investigation, 97 fresh frozen GC and their paired non-cancerous tissues were collected at Fuzhou General Hospital, which were used to assess the expression of AFAP1-AS1. The collection and preservation of fresh GC tissue samples were carried out as previously described.

4D pharma : Annual Report (SEC Filing - 20-F)

Not for trading, but in connection with the registration of American Depositary Shares, pursuant to the requirements of the Securities and Exchange Commission. Securities registered or to be registered pursuant to Section 12(g) of the Act: None None Indicate the number of outstanding shares of each of the issuer s classes of capital or common stock as of the close of the period covered by the annual report: Title of each class Ordinary Shares, nominal value £0.0025 per Share 131,467,935 Indicate by check mark if the registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act. Yes [ ] No [X]

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